The university sits on an almost perfect square of 21.5 hectares. The university transferred to its present campus in 1927 when the Dominicans deemed the Intramuros campus inadequate for the university's growing population. The first structures in the campus were the imposing Main Building, the Santisimo Rosario Parish, and the UST Gym. The Main Building and Central Seminary were declared National Cultural Treasures by the National Museum of the Philippines on January 25, 2010.
Located in front of the UST Hospital, a four-level structure wherein the first three levels consist of a multi-level carpark with operating commercial spaces in the first two levels. The UST-AMV College of Accountancy formerly occupied the fourth level of the building.
The 23-storey building is the tallest of the university's structures and the first one to have been situated outside the Manila campus. It currently houses the UST Senior High School and the College of Information and Computing Sciences.
Named after the then-Master of the Order of Preachers, Buenaventura García de Paredes. The twelve-storey building is built on the site of the original UST Gymnasium. It houses both the Faculty of Arts and Letters and College of Tourism and Hospitality Management, formerly used by Senior High School before relocating to the Frassati Building in 2019.
The eight-storey structure houses the laboratory skills facilities of the Faculty of Pharmacy, the College of Science and the College of Rehabilitation Sciences, as well as Laboratory Equipment and Supplies Office (LESO) and Research Center for Culture, Arts, and Humanities office.
The Main Building of the University of Santo Tomas declared a national treasure by the Philippine government, houses the Administration offices, the Faculty of Civil Law, the Faculty of Pharmacy, the College of Science and the Institute of Religion.
The Main Building, an academic facility, also functions as the university's administrative center. It is also the home of the Museum of Arts and Sciences.
The 28.5m x 30m four-storey building houses the university-wide student organizations such as The Varsitarian, the Central Student Council, the Office for Admission, and the Office for Student Affairs.
It an exclusive building for the library was finally inaugurated in 1990 when the UST Central Library Building was inaugurated. The six-storey library, named after the founder of the university Miguel de Benavides, is one of the biggest in Asia.
The massive edifice named after the patron saint of the university is a semi-centralized system for the productive exchange of ideas among researchers in the fields of arts, humanities, science, technology, social sciences, and education. It also houses the Graduate School and the Graduate School of Law.
The four-storey pavilion with an arena, retractable chairs, and bleachers has a seating capacity of 5,792. It serves as the new UST gymnasium that houses the varsity players of the university and the UST Growling Tigers. It also serves as a multipurpose center where graduations, university-wide activities, exhibits, national and international conferences are held. It was inaugurated in early 2012.
This building houses the UST Chapel (which is also the Santisimo Rosario Parish Church) the UST Central Seminary, and the UST Ecclesiastical Faculties. The Parish was canonically inaugurated on April 26, 1942, by Michael O'Doherty, the Archbishop of Manila during that time.
The four-story edifice, in commemoration of Miguel de Benavides' 400th death anniversary, is the first one-stop cancer therapy center in the Philippines which was inaugurated on August 21, 2006.
The UST Hospital was formally opened its charity unit on February 15, 1945, in a building which stood at the rear of the Main Building. The building house classrooms for the medical school and became the site of the first charity hospital.
On March 7, 1946, the charity hospital was opened together with UST Hospital, occupying the first floor. The whole charity ward was transferred and eventually renamed the USTH-Clinical Division when it was completed in 1965 and formally inaugurated on March 6, 1966.
Dubbed as the "concert hut", the structure houses the Conservatory of Music and was located between the UST Hospital and Roque Ruaño Building. It was demolished in 1964 to give way to the construction of Albertus Magnus Building.
The UST High School (USTHS) Building was burned down in a fire in 1975. On 1976, classes in high school were transferred to the UST Central Seminary. The new USTHS building was inaugurated in the same year and is now known as the Benavides Building.
The structure was located in the corner of Padre Noval Street and España Boulevard. It was demolished in 1990 to give way to a new school building, now known as the Beato Angelico Building. The press was renamed UST Publishing House and is currently housed in the new building.
Designed by Fernando Ocampo, the UST Gymnasium was once the largest gym in the country. It housed the Institute of Physical Education and Athletics as well as the university's swimming pool and PE annex. It was demolished to give way to the Buenaventura Garcia Paredes, O.P. Building with façade and swimming pool retained.