Percolozoa

Percolozoa
	The three different stages of N. fowleri
Scientific classification
Domain:	Eukaryota
Clade:	Diphoda
Clade:	Discoba
Superphylum:	Discicristata
Phylum:	Percolozoa; Cavalier-Smith 1991
Classes
	Heterolobosea; Lunosea; Lyromonadea; Neovahlkampfiea; Pharyngomonadea;
Synonyms
	Acrasiomycota Whittaker 1969; Amoeboflagellates; Pseudociliata Cavalier-Smith 1993; Schizopyrenia; Tetramitozoa Dillon 1963; Stephanopogonophyta Doweld 2001; Stephanopogonomorpha Sleigh et al. 1984;

The Percolozoa are a group of colourless, non-photosynthetic Excavata, including many that can transform between amoeboid, flagellate, and cyst stages.

Characteristics

Most Percolozoa are found as bacterivores in soil, fresh water and occasionally in the ocean. The only member of this group that is infectious to humans is Naegleria fowleri, the causative agent of the often fatal disease amoebic meningitis. The group is closely related to the Euglenozoa, and share with them the unusual characteristic of having mitochondria with discoid cristae. The presence of a ventral feeding groove in the flagellate stage, as well as other features, suggests that they are part of the Excavata group.

The amoeboid stage is roughly cylindrical, typically around 20–40 μm in length. They are traditionally considered lobose amoebae, but are not related to the others, and unlike them, do not form true lobose pseudopods. Instead, they advance by eruptive waves, where hemispherical bulges appear from the front margin of the cell, which is clear. The flagellate stage is slightly smaller, with two or four anterior flagella anterior to the feeding groove.

Usually, the amoeboid form is taken when food is plentiful, and the flagellate form is used for rapid locomotion. However, not all members are able to assume both forms. The genera Percolomonas, Lyromonas, and Psalteriomonas are known only as flagellates, while Vahlkampfia, Pseudovahlkampfia, and most acrasids do not have flagellate stages. As mentioned above, under unfavourable conditions, the acrasids aggregate to form sporangia. These are superficially similar to the sporangia of the dictyostelids, but the amoebae only aggregate as individuals or in small groups and do not die to form the stalk.

Terminology and classification

These are collectively referred to as schizopyrenids, amoeboflagellates, or vahlkampfids. They also include the acrasids, a group of social amoebae that aggregate to form sporangia. The entire group is usually called the Heterolobosea, but this may be restricted to members with amoeboid stages.

One Heterolobosea classification system is:^[1]

Order Schizopyrenida
- Family Vahlkampfiidae
- Family Gruberellidae
Order Acrasida
- Family Acrasidae

Pleurostomum flabellatum has recently been added to Heterolobosea.^[2]

Phylogeny

Based on the cladogram from Tolweb and updated by Pánek and Čepička 2014.^[3]^[4]

Pharyngomonada

Pharyngomonadea

Pharyngomonadida

Pharyngomonadidae	Pharyngomonas

Tetramitia

Lunosea

Selenaionida

Selenaionidae

	Selenaion

	Dactylomonas

Neovahlkampfiea

Neovahlkampfiida

Neovahlkampfiidae	Neovahlkampfia

Eutetramitia

Lyromonadea

Lyromonadida

Plaesiobystridae

	Euplaesiobystra

	Heteramoeba

Gruberellidae

Vrihiamoeba

	Oramoeba

	Stachyamoeba

Paravahlkampfiidae

	Fumarolamoeba

	Parafumarolamoeba

Paravahlkampfia

Psalteriomonadidae

	Sawyeria

	Psalteriomonas

Pseudoharpagon

Pseudomastigamoeba

	Harpagon

	Monopylocystis

Heterolobosea

Acrasida

Acrasidae

	Allovahlkampfia

	Solumitrus

	Pocheina

	Acrasis

Amoebomastigota

Naegleriida

Marinamoeba

Tulamoebidae

	Pleurostomum

	Tulamoeba

Naegleriidae

	Naegleria

	Willaertia

Vahlkampfiida s.s.

Vahlkampfiidae

	Tetramitus

	Vahlkampfia

Creneida

Creneidae	Creneis

Percolomonadida^[5]

Stephanopogonidae	Stephanopogon

Barbellidae

	Barbelia

	Nonamonas

Lulaidae

Lula

Percolomonadidae

	Percolomonas

	Nakurumonas

Taxonomy

Phylum Percolozoa Cavalier-Smith 1991^[6]

Subphylum Pharyngomonada Cavalier-Smith 1991
- Class Pharyngomonadea Cavalier-Smith 2008 [Macropharyngomonadidea]
  - Order Pharyngomonadida Cavalier-Smith 2008 [Macropharyngomonadida]
    - Family Pharyngomonadidae Cavalier-Smith 2008 [Macropharyngomonadidae Cavalier-Smith 2008]
      - Genus Pharyngomonas Cavalier-Smith 2008 [Macropharyngomonas nomen nudum]
Subphylum Tetramitia Cavalier-Smith 1993 em. Cavalier-Smith 2008
- Genus ?Costiopsis Senn 1900
- Genus ?Hoehnemastix Skvortzov 1974
- Genus ?Planiosculum Szabados 1948
- Genus ?Protomyxomyces Cunningham 1881
- Genus ?Protonaegleria Michel & Raether 1985
- Genus ?Pseudovahlkampfia Sawyer 1980
- Genus ?Schizamoeba Davis 1926
- Genus ?Tetramastigamoeba Singh & Hanumaiah 1977
- Genus ?Trimastigamoeba Whitmore 1911
- Genus ?Wasielewskia Hartmann & Schuessler 1913
- Family Euhyperamoebidae Goodkov & Seravin 1984 [Hyperamoebidae Goodkov, Seravin & Railkin 1982]
  - Genus Euhyperamoeba Goodkov & Seravin 1984 [Hyperamoeba Goodkov, Seravin & Railkin 1982 non Alexeieff 1923]
- Class Lunosea Cavalier-Smith 2021
  - Order Selenaionida Hanousková, Táborský & Čepička 2018
    - Family Selenaionidae Hanousková, Táborský & Čepička 2018
      - Genus Selenaion Park, De Jonckheere & Simpson 2012
      - Genus Dactylomonas Hanousková, Táborský & Čepička 2018
- Class Neovahlkampfiea Cavalier-Smith 2021
  - Order Neovahlkampfiida Cavalier-Smith 2021
    - Family Neovahlkampfiidae Hanousková, Táborský & Čepička 2018
      - Genus Neovahlkampfia Brown & de Jonckheere 1999
- Class Lyromonadea Cavalier-Smith 1993
  - Order Lyromonadida Cavalier-Smith 1993
    - Family Gruberellidae Page & Blanton 1985
      - Genus Gruberella Page 1984 non Gruber 1889 non Corliss 1960
      - Genus Oramoeba de Jonckheere et al. 2011
      - Genus Stachyamoeba Page 1975
      - Genus Vrihiamoeba Murase, Kawasak & Jonckheere 2010
    - Family Paravahlkampfiidae
      - Genus Fumarolamoeba De Jonckheere, Murase & Opperdoes 2011
      - Genus Parafumarolamoeba Geisen et al. 2015
      - Genus Paravahlkampfia Brown & de Jonckheere 1999
    - Family Plaesiobystridae
      - Genus Euplaesiobystra Park et al. 2009
      - Genus Heteramoeba Droop 1962
      - Genus Pernina El Kadiri, Joyon & Pussard 1992
    - Family Psalteriomonadidae Cavalier-Smith 1993 [Lyromonadidae Cavalier-Smith 1993]
      - Genus Harpagon Panek et al. 2012
      - Genus Monopylocystis O'Kelly et al. 2003
      - Genus Psalteriomonas Broers et al. 1990 [Lyromonas Cavalier-Smith 1993]
      - Genus Pseudoharpagon Panek et al. 2012
      - Genus Pseudomastigamoeba
      - Genus Sawyeria O'Kelly et al. 2003
- Class Heterolobosea Page & Blanton 1985
  - Order Acrasida Schröter 1886
    - Family Acrasidae van Tieghem 1880 ex Hartog 1906
      - Genus Acrasis van Tieghem 1880
      - Genus Allovahlkampfia Walochnik & Mulec 2009
      - Genus Pocheina Loeblich & Tappan 1961 [Guttulina Cienkowski 1873 non D'Orbigny 1839]
      - Genus Solumitrus Wang et al. 2011
  - Order Naegleriida Starobogatov 1980
    - Genus Marinamoeba De Jonckheere et al. 2009
    - Family Tulamoebidae Kirby et al. 2015
      - Genus Aurem Jhin & Park 2018
      - Genus Pleurostomum Namyslowski 1913
      - Genus Tulamoeba Park et al. 2009
    - Family Naegleriidae Kudo 1954 [Schizopyrenidae Singh 1951 ex Singh 1952; Bistadiidae Doflein 1916]
      - Genus Naegleria Aléxéieff 1912 [Adelphamoeba Napolitano, Wall & Ganz 1970; Didascalus Singh 1952; Schizopyrenus Singh 1951 ex Singh 1952]
      - Genus Willaertia de Jonckheere et al. 1984
  - Order Tetramitida Doweld 2001
    - Family Vahlkampfiidae Jollos 1917 s.s.
      - Genus Tetramitus Perty 1852 [Copromastix Aragao 1916; Learamoeba Sawyer et al. 1998, Paratetramitus Darbyshire, Page & Goodfellow 1976, Singhamoeba Sawyer, Nerad & Munson 1992]
      - Genus Vahlkampfia Chatton & LaLung-Bonnaire 1912 [Guttulidium Frenzel 1892]
  - Order Creneida Cavalier-Smith 2021
    - Family Creneidae Pánek et al. 2014
      - Genus Creneis Pánek et al. 2014
  - Order Percolomonadida Cavalier-Smith 1993
    - Family Barbeliidae Arndt 2023^[5]
      - Genus Barbelia Arndt 2021
      - Genus Nonamonas Hohlfeld, Meyer & Arndt 2023
    - Family Lulidae Hohlfeld & Arndt 2023^[5]
      - Genus Lula Arndt, Nitsche & Carduck 2021
    - Family Percolomonadidae Cavalier-Smith 1993 [Choanogasteraceae]
      - Genus Percolomonas Fenchel & Patterson 1986 [Choanogaster Pochmann 1959]
    - Family Stephanopogonidae Corliss 1961
      - Genus Nakurumonas Carduck, Nitsche & Arndt 2021
      - Genus Stephanopogon Entz 1884

History

The Heterolobosea were first defined by Page and Blanton in 1985^[7] as a class of amoebae, and so only included those forms with amoeboid stages. Cavalier-Smith created the phylum Percolozoa for the extended group, together with the enigmatic flagellate Stephanopogon.^[8]

Cavalier-Smith maintained the Heterolobosea as a class for amoeboid forms. He has defined Percolozoa as "Heterolobosea plus Percolatea classis nov."^[9]

References

^ "Heterolobosea". Retrieved 2009-03-25.
^ Park JS, Simpson AG, Lee WJ, Cho BC (July 2007). "Ultrastructure and phylogenetic placement within Heterolobosea of the previously unclassified, extremely halophilic heterotrophic flagellate Pleurostomum flabellatum (Ruinen 1938)". Protist. 158 (3): 397–413. doi:10.1016/j.protis.2007.03.004. PMID 17576098.
^ Pánek, Tomáš; Ptáčková, Eliška; Čepička, Ivan (2014). "Survey on diversity of marine/saline anaerobic Heterolobosea (Excavata: Discoba) with description of seven new species". International Journal of Systematic and Evolutionary Microbiology. 64 (Pt 7): 2280–2304. doi:10.1099/ijs.0.063487-0. PMID 24729392.
^ Cavalier-Smith T (December 2021). "Ciliary transition zone evolution and the root of the eukaryote tree: implications for opisthokont origin and classification of kingdoms Protozoa, Plantae, and Fungi". Protoplasma. 259 (3): 487–593. doi:10.1007/s00709-021-01665-7. PMC 9010356. PMID 34940909.
^ ^a ^b ^c Hohlfeld, Manon; Meyer, Claudia; Schoenle, Alexandra; Nitsche, Frank; Arndt, Hartmut (January 2023). "Biogeography, autecology, and phylogeny of Percolomonads based on newly described species". Journal of Eukaryotic Microbiology. 70 (1): e12930. doi:10.1111/jeu.12930. PMID 35712988.
^ "Part 1- Virae, Prokarya, Protists, Fungi". Collection of genus-group names in a systematic arrangement. Archived from the original on 14 August 2016. Retrieved 30 June 2016.
^ Page, F.C.; R.L. Blanton (1985). "The Heterolobosea (Sarcodina: Rhizopoda), a new class uniting the Schizopyrenida and Acrasidae (Acrasida)". Protistologica. 21: 121–132.
^ Cavalier-Smith, T. (1991). "Cell diversification in heterotrophic flagellates". In D.J. Patterson & J. Larsen (ed.). The Biology of Free-living Heterotrophic Flagellates. Oxford University Press. pp. 113–131. ISBN 9780198577478.
^ Cavalier-Smith T (November 2003). "The excavate protozoan phyla Metamonada Grassé emend. (Anaeromonadea, Parabasalia, Carpediemonas, Eopharyngia) and Loukozoa emend. (Jakobea, Malawimonas): their evolutionary affinities and new higher taxa". Int. J. Syst. Evol. Microbiol. 53 (Pt 6): 1741–58. doi:10.1099/ijs.0.02548-0. PMID 14657102.

External links

Tree of Life Heterolobosea

[urlHeterolobosea-1] "Heterolobosea". Retrieved 2009-03-25.

[pmid17576098-2] Park JS, Simpson AG, Lee WJ, Cho BC (July 2007). "Ultrastructure and phylogenetic placement within Heterolobosea of the previously unclassified, extremely halophilic heterotrophic flagellate Pleurostomum flabellatum (Ruinen 1938)". Protist. 158 (3): 397–413. doi:10.1016/j.protis.2007.03.004. PMID 17576098.

[3] Pánek, Tomáš; Ptáčková, Eliška; Čepička, Ivan (2014). "Survey on diversity of marine/saline anaerobic Heterolobosea (Excavata: Discoba) with description of seven new species". International Journal of Systematic and Evolutionary Microbiology. 64 (Pt 7): 2280–2304. doi:10.1099/ijs.0.063487-0. PMID 24729392.

[CavSmith_2021-4] Cavalier-Smith T (December 2021). "Ciliary transition zone evolution and the root of the eukaryote tree: implications for opisthokont origin and classification of kingdoms Protozoa, Plantae, and Fungi". Protoplasma. 259 (3): 487–593. doi:10.1007/s00709-021-01665-7. PMC 9010356. PMID 34940909.

[Hohlfeld_2023-5] Hohlfeld, Manon; Meyer, Claudia; Schoenle, Alexandra; Nitsche, Frank; Arndt, Hartmut (January 2023). "Biogeography, autecology, and phylogeny of Percolomonads based on newly described species". Journal of Eukaryotic Microbiology. 70 (1): e12930. doi:10.1111/jeu.12930. PMID 35712988.

[6] "Part 1- Virae, Prokarya, Protists, Fungi". Collection of genus-group names in a systematic arrangement. Archived from the original on 14 August 2016. Retrieved 30 June 2016.

[7] Page, F.C.; R.L. Blanton (1985). "The Heterolobosea (Sarcodina: Rhizopoda), a new class uniting the Schizopyrenida and Acrasidae (Acrasida)". Protistologica. 21: 121–132.

[8] Cavalier-Smith, T. (1991). "Cell diversification in heterotrophic flagellates". In D.J. Patterson & J. Larsen (ed.). The Biology of Free-living Heterotrophic Flagellates. Oxford University Press. pp. 113–131. ISBN 9780198577478.

[pmid14657102-9] Cavalier-Smith T (November 2003). "The excavate protozoan phyla Metamonada Grassé emend. (Anaeromonadea, Parabasalia, Carpediemonas, Eopharyngia) and Loukozoa emend. (Jakobea, Malawimonas): their evolutionary affinities and new higher taxa". Int. J. Syst. Evol. Microbiol. 53 (Pt 6): 1741–58. doi:10.1099/ijs.0.02548-0. PMID 14657102.

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