Nakaseomyces glabratus
| Nakaseomyces glabratus | |
|---|---|
| |
| Nakaseomyces glabratus 1600x | |
Научная классификация 
| |
| Домен: | Эукариота |
| Королевство: | Грибы |
| Разделение: | Ascomycota |
| Сорт: | Saccharomycetes |
| Заказ: | Saccharomycetales |
| Family: | Saccharomycetaceae |
| Genus: | Nakaseomyces |
| Species: | N. glabratus
|
| Binomial name | |
| Nakaseomyces glabratus (H. W. Anderson) Sugita & Takashima (2022)
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| Synonyms | |
|
Cryptococcus glabratus H.W.Anderson (1917) | |
Nakaseomyces glabratus является видом гаплоидных дрожжей Candida рода Nakaseomyces , ранее известного как glabrata . Несмотря на то, что для этого вида не было задокументировано никакого сексуального жизненного цикла, штаммы N. glabratus обоих типов спаривания обычно встречаются. [ 1 ] N. glabrata, как правило, является комменсалом тканей слизистой оболочки человека, но в сегодняшнюю эпоху более широкого иммунодефицита человека по различным причинам (например, терапевтическая иммуномодуляция , более длительная выживаемость с различными сопутствующими заболеваниями, такими как диабет и ВИЧ -инфекция ), N. Часто является Вторая или третья наиболее распространенная причина кандидоза как оппортунистического патогена . [ 2 ] Инфекции, вызванные N. glabratus, могут влиять на урогенитальный тракт или даже вызывать системные инфекции путем входа в грибные клетки в кровоток ( кандидемия ), особенно распространенные у пациентов с иммунитетом. [ 2 ]
Клиническая значимость
[ редактировать ]N. glabratus имеет особое значение при носокомиальных инфекциях из -за его врожденной высокой устойчивости к противогрибковым агентам, в частности, азолы . [ 2 ] Помимо его врожденной толерантности к противогрибковым препаратам, другие потенциальные факторы вирулентности способствуют N. glabratus патогенности . Одним из них является экспрессия серии генов адгезинов . [3] These genes, which in N. glabratus are mostly encoded in the subtelomeric region of the chromosome, have their expression highly activated by environmental cues, so that the organism can adhere to biotic and abiotic surfaces in microbial mats. Adhesin expression is the suspected first mechanism by which N. glabratus forms fungal biofilms, proved to be more resistant to antifungals than the planktonic cells.[4]
N. glabratus genome frequently undergoes rearrangements that are hypothesized to contribute to the improvement of this yeast's fitness towards exposure to stressful conditions, and some authors consider that this property is connected to the virulence potential of this yeast.[5]
Diagnosis
[edit]
Cultures are an effective method for identifying non-albicans vaginal infections. Urinalyses are less accurate in this process. The culture may take several days to grow, but the identification of the yeast species is quick once the yeast is isolated. Skin disease diagnosis is difficult, as cultures collected from swabs and biopsies will test negative for fungus and a special assessment is required. Listed under the 'Rare Diseases' database on the NIH web site[verification needed], Torulopsis glabrata, or N. glabratus can also be found on the CDC's web site.[6] Although listed as the second most virulent yeast after Candida albicans, the fungus is becoming more and more resistant to common treatments like fluconazole. N. glabratus resistance to Echinocandin is also increasing, leaving expensive and toxic antifungal treatments available for those infected.[6] Although high mortality rates are listed, assessment of the critical nature of a glabrata infection is a gray area.
N. glabratus ferments and assimilates only glucose and trehalose, opposing to Candida species and this repertoire of sugar utilization is used by several commercially available kits for identification.[7]
Treatment
[edit]A major phenotype and potential virulence factor that N. glabratus possesses is low-level intrinsic resistance to the azole medications, which are the most commonly prescribed antifungal (antimycotic) medications. These medications, including fluconazole and ketoconazole, are "not effective in 15–20% of cases"[2] against N. glabratus. It is still highly vulnerable to polyene medications such as amphotericin B and nystatin, along with variable vulnerability to flucytosine and caspofungin. However, intravenous amphotericin B is a medication of last resort, causing among other side effects, chronic kidney failure. Amphotericin B vaginal suppositories are used as an effective form of treatment in combination with boric acid capsules as they are not absorbed into the bloodstream.
A first-line treatment for vaginal infections may be the use of terconazole 7-day cream. Several courses may be needed. The cure-rate for this treatment is approximately 40%. Recurrences are common, causing chronic infections and spread to other areas such as skin and scalp. Blood infections might be best assessed per symptoms if other areas are involved.
An experimental, but effective second-line treatment for chronic infections, is the use of boric acid. Compounding pharmacies can create boric acid vaginal suppositories. Use of Vitamin E oil may be used in conjunction to combat irritation. Amphotericin B vaginal suppositories have also been used in case studies to treat chronic infections, both symptomatic and asymptomatic. Borax and boric acid may be used for persistent scalp and skin infections.
Phylogenetic relationship
[edit]N. glabratus is more closely related to Saccharomyces cerevisiae than to Candida species. In fact, N. glabratus belongs to the group of Nakaseomyces inside the whole genome duplication clade within Saccharomycetaceae.[8] The whole genome duplication event occurred about 90 million years ago, whereas phylogenetic studies indicate that the common ancestor between N. glabratus and C. albicans is dated between 200 and 300 million years ago. The largest phylogenetic study to date about Saccharomycotina, also known as budding yeasts, indicated in 2018 that the (currently construed) genus Candida is found in Pichiaceae, CUG-Ser1 clade, Phaffomycetaceae and Saccharomycetaceae. Consequently, despite that the name Candida evokes a unitary notion of candidiasis, the pathogenic power of some budding yeasts is a paraphyletic trait shared by several subphyla with different kinds of metabolism.[9]
References
[edit]- ^ Fairhead, Cécile; Dujon, Bernard; Gallaud, Julien; Hennequin, Christophe; Muller, Héloïse (2008-05-01). "The Asexual Yeast Candida glabrata Maintains Distinct a and α Haploid Mating Types". Eukaryotic Cell. 7 (5): 848–858. doi:10.1128/EC.00456-07. ISSN 1535-9778. PMC 2394967. PMID 18375614.
- ^ Jump up to: a b c d Fidel, Paul L.; Vazquez, Jose A.; Sobel, Jack D. (1999). "Candida glabrata: Review of Epidemiology, Pathogenesis, and Clinical Disease with Comparison to C. albicans". Clinical Microbiology Reviews. 12 (1): 80–96. doi:10.1128/CMR.12.1.80. ISSN 0893-8512. PMC 88907. PMID 9880475.
- ^ Michael, de Groot, Piet W. J. Kraneveld, Eefje A. Yin, Qing Yuan Dekker, Henk L. Groß, Uwe Crielaard, Wim de Koster, Chris G. Bader, Oliver Klis, Frans M. Weig. The Cell Wall of the Human Pathogen Candida glabrata: Differential Incorporation of Novel Adhesin-Like Wall Proteins ▿ †. American Society for Microbiology (ASM). OCLC 677694114.
{{cite book}}: CS1 maint: multiple names: authors list (link) - ^ Cavalheiro, Mafalda; Teixeira, Miguel Cacho (2018). "Candida Biofilms: Threats, Challenges, and Promising Strategies". Frontiers in Medicine. 5: 28. doi:10.3389/fmed.2018.00028. ISSN 2296-858X. PMC 5816785. PMID 29487851.
- ^ Ahmad, Khadija M; Kokošar, Janez; Guo, Xiaoxian; Gu, Zhenglong; Ishchuk, Olena P; Piškur, Jure (June 2014). "Genome structure and dynamics of the yeast pathogen Candida glabrata". FEMS Yeast Research. 14 (4): 529–535. doi:10.1111/1567-1364.12145. ISSN 1567-1356. PMC 4320752. PMID 24528571.
- ^ Jump up to: a b "Antifungal Resistance". Fungal Diseases. Centers for Disease Control and Prevention. 22 June 2017.
- ^ Bonnin, Alain; Vagner, Odile; Cuisenier, Bernadette; Pacot, Agnès; Nierlich, Anne-Charlotte; Moiroux, Philippe; Mantelin, Pierre; Dalle, Frédéric; Lopez, José (2001-03-01). "Rapid Identification of Candida glabrata Based on Trehalose and Sucrose Assimilation Using Rosco Diagnostic Tablets". Journal of Clinical Microbiology. 39 (3): 1172–1174. doi:10.1128/JCM.39.3.1172-1174.2001. ISSN 0095-1137. PMC 87898. PMID 11230452.
- ^ Gabaldón, Toni; Martin, Tiphaine; Marcet-Houben, Marina; Durrens, Pascal; Bolotin-Fukuhara, Monique; Lespinet, Olivier; Arnaise, Sylvie; Boisnard, Stéphanie; Aguileta, Gabriela; Atanasova, Ralitsa; Bouchier, Christiane (2013-09-14). "Comparative genomics of emerging pathogens in the N. glabratus clade". BMC Genomics. 14 (1): 623. doi:10.1186/1471-2164-14-623. ISSN 1471-2164. PMC 3847288. PMID 24034898.
- ^ Shen, Xing-Xing; Opulente, Dana A.; Kominek, Jacek; Zhou, Xiaofan; Steenwyk, Jacob L.; Buh, Kelly V.; Haase, Max A. B.; Wisecaver, Jennifer H.; Wang, Mingshuang; Doering, Drew T.; Boudouris, James T. (2018-11-29). "Tempo and Mode of Genome Evolution in the Budding Yeast Subphylum". Cell. 175 (6): 1533–1545.e20. doi:10.1016/j.cell.2018.10.023. ISSN 0092-8674. PMC 6291210. PMID 30415838.